Intshayelelo: Ukuguqulwa koMphezulu woMbane kunye nokuChaneka koDiagnostic
Kumhlaba oguqukayo wobomi besayensi kunye noxilongo, ukudityaniswa kwesixhobo esikhethekileyo kukuhanjiswa komsebenzi olungelelanisiweyo kubaluleke kakhulu ekwandiseni ukuveliswa kophando kunye nokukhawulezisa ukufunyanwa. Unyango lwe-corona yelabhoratri, isixhobo esenzelwe ukusebenza ngqo kumphezulu ngokukhutshwa kombane, ngokwesiko sanxulunyaniswa nenzululwazi yemathiriyeli kunye neenkqubo zoshishino. Nangona kunjalo, isakhono sayo phakathi kwale mihla, idata{2}}imimandla yelabhoratri eqhutywayo{3}}ingakumbi ngokudibana novavanyo oluphambili lwebhayoloji kunye neselula-ihlala ingumda onyanzelisayo wokutshintsha.
Indima yoNyango lweCorona kuLungiselelo lweSampuli esiSiseko
Embindini wayo, umnyangi we-corona yelabhoratri uguqula amandla angaphezulu ngokufaka -ukukhutshwa kwe-voltage ephezulu kwizinto, inkqubo ebalwa ngeeparamitha ezifana namandla (W) angumsebenzi wenqanaba lonyango, isantya, ububanzi, kunye neeparamitha zomatshini. Oku kulawulwa komphezulu wokwenziwa kusebenze kulinyathelo elibalulekileyo, kodwa elihlala lingahoywa, linyathelo lokulungiselela uninzi lokuhamba komsebenzi we-bioanalytical. Umzekelo, ekukhuliseni nasekuhlalutyweni kweeseli zebhaktiriya okanye zomntu, ukumanzisa kwesubstrate okungaguqukiyo kunye nokuncamathela-akuxoxiswana ngokuthembeka kovavanyo. Nokuba ulungiselela imiphezulu yepolymer yokwenziwa kwesixhobo se-microfluidic ukugquma iibhaktheriya kumathontsi e-nanoliter, okanye ukunyanga i-cultureware yokuphucula ukuncamatheliswa kweeseli zethumba lesibindi somntu kwiijeli ze-biomimetic, unyango lwe-corona luqinisekisa iimeko zokuqala ezifanayo. Oku kubekwa emgangathweni kwindawo yolungiselelo lwesampulu kukhulisa ngokuthe ngqo ukunyaniseka komgangatho osezantsi,-uhlalutyo lwemveliso ephezulu.
Ukudityaniswa ne-Advanced Diagnostic and Monitoring Workflows
Ixabiso lokwenyani lonyango lwe-corona yelabhoratri livulwa ngokudityaniswa kwayo ngokungenamthungo kwiinkqubo ezibanzi, ezidityanisiweyo zelebhu. Qwalasela uhambo lomsebenzi wovavanyo lokuchaphazeleka ngamayeza okubulala iintsholongwane. Inkqubo inokuqala nge-corona{2}}indawo enyangweyo iqinisekisa ukwakheka kwethontsi yeseli enye{3}}yebhaktiriya encapsulation. Ukulandela ukufukanyelwa, iimvavanyo zokuxilonga ngokukhawuleza, ezifana nelatex agglutination yokuchonga iStreptococcus pneumoniae okanye ukufumanisa iMethicillin{5}}I-Resistant Staphylococcus aureus (MRSA), iyenziwa. Unyango lokuqala lomphezulu lunegalelo ekusebenzeni okuhambelanayo kwezi zixhobo ezikhawulezayo ngokuqinisekisa ukuba ii-reagents zisebenzisana ngokufanayo kunye neepleyiti eziphathwe kakuhle okanye indawo yesilayidi. Ngaphaya koko, kwiseli -uvavanyo olusekwe njengokulinganisa ukusasazwa kwechiza{9}apho iiseli zigcinwa kwimidiya eyongezwa ngeserum yenkomo yomntwana kunye ne-antibiotics{10}}imikhumbi ye-corona{11}}enyangwayo yenkcubeko ikhuthaza i-homogeneous cell monolayers, ekhokelela kumfanekiso ophindaphindwayo kunye nobungakanani bedatha. Oku kudala ikhonkco elidibeneyo ukusuka ekulungiseleleni isampuli ukuya kuhlalutyo, ukunciphisa iinguqu ezivezwe ngokungahambelani kwe-substrate.
Ukuqhuba iNguqu yeDijithali kunye nokuphucula amava oLuntu
Ukudibanisa izixhobo ezifana nonyango lwe-corona kudlula nje ukubekwa kokuhamba komsebenzi ngokwasemzimbeni; yinxalenye ephambili yenguqu yedijithali yelebhu. Ilebhu yanamhlanje ijolise ekudibaniseni yonke imiba yokusebenza, ukusuka kwizixhobo kunye nezinto ezisetyenziswayo ukuya ekuhambeni komsebenzi kunye nedatha. Umphathi we-corona okrelekrele, oqhagamshelweyo unokungena kwiiparamitha zonyango (umzekelo, Amandla (W), ixesha lokuvezwa) ngokuthe ngqo kwincwadi yamanqaku yelebhu yedijithali okanye iNkqubo yoLawulo loLwazi lweLebhu (LIMS). Oku kudala umkhondo onokuphicothwa kumanyathelo okulungiselela abalulekileyo, ukuqinisekisa ukuthotyelwa kunye nokuveliswa kwakhona. Xa le datha idityaniswe neziphumo ezisuka kumanyathelo alandelayo{6}}ezifana neemetrics zokukhula kwebhaktheriya ukusuka ekujongweni kwe-droplet okanye idatha yokuthatha iselula kwizifundo zokucinga{7}}izazinzulu zinokufumana ulwazi olunzulu ngohlalutyo oluphambili. Olu qhagamshelo lunciphisa iimpazamo zoshicilelo ngesandla, ixesha lokungasebenzi kwesixhobo, kwaye kwandisa ukusetyenziswa kwezibonelelo zaselabhoratri. Ekugqibeleni, ngokuzenzekela kunye nokubhala inyathelo lokulungiselela umphezulu, itekhnoloji inciphisa imisebenzi yezandla edinayo, ivumela oosonzululwazi ukuba bagxile{10}}kuhlahlelo oluphezulu lwexabiso kunye nokwenza izigqibo{11}}. Oku kuhambelana nenjongo yokuphucula amava omntu kwilebhu, ukwenza ukuhamba komsebenzi kusebenze ngakumbi, kuthembekile, kwaye kubandakanyeke ngokwasengqondweni.
Isiphelo kunye neembono zexesha elizayo
Ukudityaniswa konyango lwe-corona yelabhoratri kwiinkqubo zovavanyo ezintsonkothileyo kubonisa ukudibana kobunjineli obuchanekileyo, i-microbiology, kunye nolawulo lwelebhu yedijithali. Ngokubonelela ngendlela eyomeleleyo yokumisela iimpawu zomgangatho ophezulu, ixhasa ukuthembeka kokusika{1}}ubuchule bomda ukusuka kwi{2}}kuhlalutyo lwebhaktiriya yeseli ukuya kwiimodeli ezintsonkothileyo zenkcubeko yeseli ye-3D. Njengoko iilabhoratri ziqhubeka nokuguqukela kwimekobume enobuchule, eqhagamshelene ngakumbi, indima yobuchwephesha bokwenza obu buchule iya kukhula ngokubaluleka. Uphuhliso lwexesha elizayo lungabona abenzi becorona abakhelwe{6}ngaphakathi abenzi boluvo bokwenyani{7}}kwexesha loqinisekiso lwamandla omphezulu, okanye nkqu nodibaniso olungqingqwa kunye neenkqubo zokuphatha ulwelo oluzenzekelayo, ukuvala ngakumbi umjikelo phakathi kolungiselelo lwesampulu, uhlalutyo, kunye nobhaqo{8} oluqhutywayo. Ukwamkela olu hlanganiso ayilophuculo lobugcisa kuphela kodwa linyathelo elicwangcisiweyo lokwakha amashishini omelele ngakumbi, anemveliso, kunye nanentshayelelo yobunzululwazi.